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. 1990 Jun 25;18(12):3489–3493. doi: 10.1093/nar/18.12.3489

Three genes under different developmental control encode elongation factor 1-alpha in Xenopus laevis.

M K Djé 1, A Mazabraud 1, A Viel 1, M le Maire 1, H Denis 1, E Crawford 1, D D Brown 1
PMCID: PMC331001  PMID: 2362804

Abstract

We have cloned cDNAs encoding two variants of the elongation factor for protein synthesis in Xenopus laevis, called EF-1 alpha. One of these (42Sp50) is expressed exclusively in immature oocytes. It is one of two protein components of a 42S RNP particle that is very abundant in previtellogenic oocytes. The 42S RNP particle consists of various tRNAs, 5S RNA, 42Sp50 and a 5S RNA binding protein (42Sp43). A major function served by 42Sp50 appears to be the storage of tRNAs for later use in oogenesis and early embryogenesis. The second EF-1 alpha variant (EF-1 alpha O) is expressed mainly in oocytes but transiently in early embryogenesis as well. Its mRNA cannot be detected after neurulation in somatic cells. EF-1 alpha O is closely related to a third EF-1 alpha (EF-1 alpha S), discovered originally by Krieg et al. (1). EF-1 alpha S is expressed at low levels in oocytes but actively in somatic cells. The latter two proteins are very similar to known eukaryotic EF-1 alpha from other organisms and presumably function in their respective cell types to support protein synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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