Figure 4. Boosting with serologically distinct recombinant virus increases protective potency of the effector response.

A) Prime-boost and challenge strategy. B) Lesion scores in mice primed with PR8-LACK_ins and boosted with X31-LACK_ins (PR8/X31); and PBS controls. C) Parasite burdens in PR8/X31 vaccinated mice and PBS controls (n = 3 for wk 2 and 6, n = 5 for wk 11), *p = 0.009. D) Cytokine production following challenge. Spleens and dNL from vaccinated and control mice were assessed by ex vivo ICS for IFN-γ production. Mean, pooled data ± SEM are plotted (n = 3 for non-challenged mice, n = 5 for wk 2 and 6); *p = 0.02, **p = 0.03, ***p = 0.005, ****p = 0.001, #p = 0.02, ##p = 0.002, ###p = 0.03. Cells have been gated on CD4⁺ CD44⁺.