Table 2. In vitro susceptibility and Fksp mutations of 20 echinocandin-resistant Candida spp. isolates, France, 2004–2010.
| Patient no. |
Strain |
Species |
MIC, µg/m, AM3/RPMI 1640 medium |
|
Fksp mutation | |||
|---|---|---|---|---|---|---|---|---|
| Caspofungin |
Micafungin |
Anidulafungin |
Gene |
Mutation |
||||
| 1* | 05BL1-38 | C. albicans | 1/2 | 0.25/1 | 0.06/0.125 | FKS1 (HS1) | F641S | |
| 2* | ODL13-1254 | C. albicans | 1/2 | 1/1 | 0.5/0.5 | FKS1 (HS1) | S645Y | |
| 3† | 06BL2-127 | C. albicans | 2/2 | 1/0.5 | 0.125/0.125 | FKS1 (HS1) | F641S‡ + S645P‡ | |
| 4 | ODL19-1894 | C. albicans | 4/2 | 2/2 | 0.125/0.25 | FKS1 (HS1) | S645P | |
| 5* | 08BL1-94 | C. albicans | 2/4 | 0.25/1 | 0.06/0.5 | FKS1 (HS2) | R1361G§ | |
| 6* | 08BL2-143 | C. albicans | 8/4 | 4/2 | 0.25/0.5 | FKS1 (HS1) | S645P | |
| 7* | 09BL1-43 | C. albicans | 1/2 | 0.25/1 | 0.06/0.25 | FKS1 (HS1) | F641S | |
| 8* | 09BL1-77 | C. albicans | 0.5/0.5 | 0.5/0.25 | 0.015/0.03 | FKS1 (HS1) | R647G§ | |
| 9 | 06BL1-34 | C. krusei | 4/8 | 2/4 | 1/2 | FKS1 (HS1) | L648W§,¶ | |
| 10* | 10BL1-50 | C. krusei | 2/4 | 1/2 | 0.06/1 | FKS1 (HS1) | F645L§,¶ | |
| 11 | ODL7-647 | C. glabrata | 8/8 | 0.5/1 | 0.25/0.125 | FKS2 | DelF658# | |
| 12* | 07BL2-157 | C. glabrata | 4/1 | 1/0.5 | 0.25/0.5 | FKS2 | DelF658# | |
| 13* | 06BL1-33 | C. glabrata | 8/8 | 4/8 | 2/2 | FKS2 | S663P | |
| 14* | ODL21-2028 | C. glabrata | 1/1 | 0.25/0.25 | 0.25/0.25 | FKS1 | S629P | |
| 15* | ODL22-2183 | C. glabrata | 8/2 | 0.25/0.25 | 0.25/1 | FKS2 | S663P | |
| 16 | ODL23-2221 | C. glabrata | 1/4 | 0.06/2 | 0.06/0.25 | FKS1 + FKS2 | F625I§ (FKS1) + P667T§ (FKS2) | |
| 17* | 08BL2-142 | C. glabrata | 1/4 | 0.25/2 | 0.25/2 | FKS2 | S663P | |
| 18 | 09BL1-55 | C. glabrata | 8/4 | 2/4 | 0.5/0.5 | FKS2 | S663P | |
| 19 | 10BL1-19 | C. glabrata | 0.5/4 | 0.06/0.5 | 0.06/1 | FKS2 | F659S + L664V§ | |
| 20 | 10BL1-67 | C. glabrata | 4/4 | 0.5/1 | 0.125/1 | FKS2 | DelF658# | |
*Parentage of initial isolate available. †In this patient, another isolate with reduced susceptibility to echinocandin was retrieved. This isolate harbored an S645P mutation in FKS1. ‡Heterozygous mutation. §Mutations not already described (13). ¶Strains had also an L701M mutation. #Deletion.