Table. Primers and probe used for diagnostic PCR and primers used to sequence Saffold virus, 2009*.

Assay, primer	Type	Sequence, 5′ → 3′
Diagnostic RT-PCR
Saffold F	Forward primer	CTA WCA TGC CTC CCC GAT T
Saffold R	Reverse primer	GYT TAG ACC GGG GGA ACC
Saffold Probe	Probe	TTT CTG CCC TGC TGG GCG G
VP2 typing
SAFV VP2 OF	Outer forward primer	GAR ATG ACY AAY CTB TCW GAY AGA GT
SAFV VP2 OR	Outer reverse primer	CCR TTR AAN ACS GGY TTN AC
SAFV VP2 IF	Inner forward primer	CGG CYA YAA ACA CKC AAT C
SAFV VP2 IR	Inner reverse primer	TTD GCR TGY TGN GTC CA
VPI typing, type 2 specific
SAFV-2 VP1 OF1	Outer forward primer 1	GCA GAA AAA GGA AAG GTT GC
SAFV-2 VP1 OF2	Outer forward primer 2	GCA GAA AAA GGC AAA GTT GC
SAFV-2 VP1 OR	Outer reverse primer	TCT TGG RCA AAA CAC TCT CA
SAFV-2 VP1 IF	Inner forward primer	CYA TAG CTC TTC CTG AAA AYC A
SAFV-2 VP1 IR	Inner reverse primer	TGR ACC GAA AAY CTG TCT GC

*RT-PCR, real-time reverse transcription PCR; SAFV, Saffold virus: VP, viral protein.