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. 2012 Jan;18(1):142–145. doi: 10.3201/eid1801.111092

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Western blot analysis of protease-resistant prion protein in the brain (thalamus/hypothalamus) and spleen of mouse lemurs inoculated with a cattle-derived L-type bovine spongiform encephalopathy (BSE) isolate by oral and intracerebral routes by using SHa31 monoclonal antibody against prion protein. Lanes 1, 7: cattle L-type BSE isolate (02-2528); lanes 2, 3: brain sample from intracerebral inoculation at 5 mg; lane 4: brain sample from oral inoculation at 50 mg; lanes 5, 6: brain sample from oral inoculation at 5 mg; lanes 8, 9: spleen samples from oral inoculation at 5 mg, positive and negative, respectively.