Fig. 2.

BCG, M. vaccae and M. obuense enhance γδ T-cell cytotoxicity. PBMCs were cultured overnight with heat-killed BCG, M. vaccae (Mv) and M. obuense (Mo). Untreated (un) cells were used as a negative control. PBMCs were then co-cultured for 6 h with Daudi or A549 cells at an effector:target cell ratio of 2:1 and CD107a/b expression measured within gated Vδ2⁺ cells. a Mean percentage of Vδ2⁺ cells expressing CD107a/b in the absence (media) or presence of Daudi or A549 target cells (n ≥ 5). b Representative flow cytometric dot plots from one donor showing CD107a/b expression on gated Vδ2⁺ cells. c Mean percentage of Vδ2⁺ cells expressing CD107a/b in the absence (Media) or presence of Daudi target cells (n = 5). d Media test scores were subtracted from Daudi test scores. Mean values are shown for n = 5. Error bars represent SD and * indicates a P value of <0.05 for statistical comparisons between untreated and treated conditions. For statistical testing, data were standardised by subtracting the untreated scores from test scores. e Percentage of Vδ2⁺ cells expressing CD107a/b and mean fluorescent intensity (MFI) expression of CD107a on Vδ2⁺ cells in the presence of zoledronic acid-treated A549 cells. Background levels of degranulation were subtracted from A549-induced degranulation. Individual experiments for three donors are shown