Fig. 5.

IL-12, IL-1β and TNF-α induce γδ T-cell IFN-γ production and cytotoxicity. a γδ T-cells were cultured overnight with different concentrations and combinations of IL-12, IL-1β and TNF-α prior to measuring IFN-γ expression on gated Vδ2⁺ and Vδ2⁻ cells. Individual experiments from three donors are shown. b MFIs for granzyme B expression on gated γδ T-cells after overnight culture with 10 ng/ml each of IL-12, IL-1β and TNF-α. Left: Representative flow cytometric histogram plots from one donor. Black fill is the isotype control antibody. Numbers are MFIs within the marker shown. Right: MFI values for n = 3. c Cytokine-activated γδ T-cells were co-cultured overnight with Daudi cells at the target (T) to effector (E) cell ratios shown. Cytotoxicity was assessed by measuring lactate dehydrogenase release into the culture supernatants. Mean values for n = 3 (T:E 1:50) and n = 2 (T:E 1:10 and 1:20) are shown. Untreated (un) cells were used as a negative control throughout. Error bars represent SD deviations and * indicates a P value of <0.05 for paired t tests between treatments at the T:E ratio of 1:50