Fig. 1.
(A) GISP increases levels of GPCRs and ion-channel receptors. A, GISP increases levels of GABAB2, CaSR, GluR1, GluR2 and GluR6 receptors in HEK293 cells. HEK293 cells were transfected with the receptor plasmid together with either a control vector (pCAT) or vector encoding GISP. 48 h post-transfection, the total receptor protein was determined by Western blotting with anti-receptor antibodies. The doublets for CaSR are due to mature (glycosylated) and immature (non-glycosylated) receptors. Immunoblots were probed with anti-β-actin antibody to ensure equal loading. (B) The bar charts show densiometric analysis of at least three blots and the dashed line indicates the control expression of each receptor (mean ± SEM). In each case, receptor protein expression was normalised to actin. The increase in receptor expression when co-transfected with GISP is significant *p < 0.01; **p < 0.001.