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. Author manuscript; available in PMC: 2012 Mar 22.
Published in final edited form as: Neurosci Lett. 2009 Jan 13;452(2):106–110. doi: 10.1016/j.neulet.2009.01.011

Fig. 2.

Fig. 2

Effect of GISPΔ8-Mut protein on neurotransmitter receptor expression assessed by immunoblotting. (A) GISPΔ8-Mut does not increases levels of neurotransmitter receptors, apart from GluR1, in HEK293 cells compared to control pCAT. HEK293 cells were transfected with the receptor plasmid together with either a control vector (pCAT) or GISPΔ8-Mut. 48 h post-transfection, the total receptor protein was determined by immunoblotting and blots were probed with anti-receptor antibodies. Immunoblots were probed with anti-β-actin antibody to ensure equal loading and normalisation. (B) The bar charts show normalised densiometric analysis of three blots. The receptor expression when co-transfected with GISPΔ8-Mut is not significant compared to control, apart from in the case of GluR1 (*p < 0.01). (C) GISP does not increases levels of transferin receptor in HEK293 cells compared to GISP mutant. HEK293 cells were transfected with either a control vector (pCAT) or vector encoding GISP or GISPΔ8-Mut. 48 h post-transfection, the total receptor protein was determined by immunoblotting and blots were probed with anti-TfR antibodies.

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