Figure 3.

Effect of berberine on human preadipocyte differentiation. Cells differentiated in the absence or presence of different concentrations of berberine over 16 days. The degree of differentiation was determined by Oil-Red-O staining. (a) Photomicrographs representing cells maintained in different concentrations of berberine: (A) control, (B) 0.1 μM berberine, (C) 1 μM berberine, (D) 10 μM berberine, ×100. (b) Absorbance value representing the mean ± SEM of the three separate experiments in six replicates. *P < 0.01, comparison between berberine-treated groups and control group, ^† P < 0.05, comparison among berberine-treated groups.