Skip to main content
. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Biol Psychiatry. 2011 Sep 13;70(11):1039–1048. doi: 10.1016/j.biopsych.2011.07.032

Figure 4.

Figure 4

Downregulation of 5-HT1BRs in the OFC following chronic treatment with SRIs. 5-HT1B receptor autoradiography using [125I]-CYP was performed on coronal brain slices from mice treated for 4 weeks with control (n= 13), 10 mg/kg/d fluoxetine (n=13) (B), 20 mg/kg/d clomipramine (n=12) (C), or 20 mg/kg/d desipramine (n=12) (D). Representative autoradiograms showing [125I]-CYP binding in OFC and DFC of control- and fluoxetine-treated mice (A). Nonspecific [125I]-CYP binding was determined in the presence of the 5-HT1B/1DR antagonist GR127935 (20 μm) and was subtracted from total binding to give specific binding. All data are expressed as percent of control-treated specific binding. OFC, orbitofrontal cortex; DFC, dorsofrontal cortex; CPu, caudate-putamen; NAc, nucleus accumbens. Results are presented as means ± s.e.m. Asterisk (*) indicates P <0.05 compared to saline.