Effect of MB-TIM fractions on the glucose uptake of liver cells. The TIM fractions were collected in 1 h intervals during a 4 h digestion period. Glucose uptake of H4IIE cells was measured after treatment with MB-TIM fractions at 100 μg/mL, insulin at 50 nM or vehicle (Control). Glucose uptake was measured according to the protocols described in Materials and Methods. The experiment is representatitive of a series of experiments and values are the mean of three replicates +/− SD, **: p<0.05 vs control, ***: p<0.01 vs control, ****: p<0.001 vs initial, One-way ANOVA, Bonferroni’s Multiple Comparison Test