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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Prostate. 2011 Dec 7;72(10):1104–1116. doi: 10.1002/pros.22457

Fig. 1.

Fig. 1

PEITC treatment inhibits cell growth and induces apoptosis through down -regulation of XIAP and Survivin proteins in cultured human prostate cancer cells. A: Effect of PEITC treatment on viability of PC -3 and LNCaP. The cells were treated for specified time periods with DMSO (control)or the indicated concentrations of PEITC and then number of viable cells w as determined by trypan blue dye exclusion assay. Results shown are mean ±SD ( n=3). Significantly different (P< 0.05) compared with acorresponding DMSO-treated control by one-way ANOVA followed by Dunnett’s test. B: Effect of PEITC treatment on histone-associated apoptotic DNA fragment release into the cytosol in PC-3 and LNCaP cells. The cells were treated for specified time points with DMSO (control) or the indicated concentrations of PEITC. Apoptosis enrichment relative to corresponding DMSO-treated control is shown. Results shown are mean ±SD ( n=3). Significantly different (P <0.05) compared with acorresponding DMSO-treated control by one-way ANOVA followed by Dunnett’s test. C: Western blotting for XIAP, Survivin, and cIAP2 proteins using lysates from PC-3 and LNCaP cells treated with DMSO (control) or the indicated concentrations of PEITC for specified time periods. Blots were stripped and reprobed with anti-actin antibody to correct for differences in protein loading. Numbers on top of bands are fold changes in expression relative to corresponding DMSO-treated control. D: RT -PCR for XIAP and Survivin mRNA expression in PC-3 and LNCaP cells treated with DMSO (control) or the indicated concentrations of PEITC for specified time periods. Numbers above bands represent change in mRNA levels relative to corresponding DMSO-treated control. Each experiment was repeated at least twice.