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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Prostate. 2011 Dec 7;72(10):1104–1116. doi: 10.1002/pros.22457

Fig. 5.

Fig. 5

Survivin overexpression confers protection against PEITC-induced apoptosis in human prostate cancer cells. A: Immunoblotting for Survivin and full-length PARP using lysates from PC-3 and LNCaP cells transiently transfected with empty vector (abbreviated as PC-3/EV and LNCaP/EV) or vector encoding for Survivin (abbreviated as PC-3/Survivin and LNCaP/Survivin) and treated for 24 hours with DMSO (control) or the indicated concentrations of PEITC. Numbers above bands represent change in protein levels relative to empty vector-transfected cells treated with DMSO. Effect of PEITC treatments on (B)cell viability and (C)histone -associated DNA fragment release into the cytosol in PC-3 and LNCaP cells transiently transfected with empty vector or vector encoding for Survivin and treated for 24 hours with DMSO or the indicated concentrations of PEITC. Results shown are mean ±SD (n=3)and normalized to corresponding DMSO-treated control. Significantly different (P< 0.05) compared with acorresponding DMSO-treated control and bbetween empty vector-transfected cells and Survivin overexpressing cells at each dose (0, 2.5, and 5 μM PEITC) by one-way ANOVA followed by Bonferroni’s multiple comparison test. Each experiment was repeated at least twice, and representative data from one such experiment are shown.