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. Author manuscript; available in PMC: 2013 Mar 20.
Published in final edited form as: Anal Chem. 2012 Mar 2;84(6):2862–2867. doi: 10.1021/ac203394r

Figure 1. Peptide recovery after SDS removal.

Figure 1

A Shewanella whole cell lysate was digested by the urea digestion protocol and peptides were cleaned up by C-18 SPE column before use. 20 μg of peptides were used in each experiment. A, peptide recovery after treatment with 2M KCl, 1% SDS, or 1% SDS followed by SDS removal via KDS precipitation. B, peptide recovery after SDS removal and C-18 SPE clean up. C, peptide recovery from different available SDS removal protocols. All data were summarized from triplicates. #, below the detect limitation.