Skip to main content
. 2012 Mar 7;2012:984024. doi: 10.1155/2012/984024

Figure 5.

Figure 5

Effect of XCHT on free radicals in glucose-exposed RMCs. (a) Direct free radical scavenging activity of XCHT determined by the DPPH radical scavenging assay. Values represent the data of three independent experiments (SE < 0.05). Vitamin C was used as a positive control compound. (b) Intracellular visualization of ROS in RMCs under different treatments using DHE stains; (A) normal-glucose medium, (B) high-glucose medium, (C) high-glucose medium containing XCHT (20 μg/mL), (D) high-glucose medium containing tiron (antioxidant control; 10 mmol/L). (c) Quantitation of ROS generation in RMCs using CM-H2DCFDA assay. RMCs were cultured in normal glucose (NG; 5 mmol/L) or high-glucose (HG; 30 mmol/L) medium in the presence of XCHT (1, 10, 20, and 50 μg/mL) or tiron (10 mmol/L) for 24 h. # P < 0.05 compared to NG., *P < 0.05 compared to HG.