Table 3. Dose-dependent effects of acute alcohol on p-ERK1/2 immunoreactivity 30- minutes after injection in adolescent and adult mouse brain.

Data are shown as percent change in mean ± SEM p-ERK1/2 IR as compared to parallel aged-matched saline controls at each dose of ethanol tested (n=8 per condition). * - indicates significantly different from corresponding saline control, P≤0.05. Values in bold text indicate adolescent-specific changes.

Age	Brain regions	0.5 g/kg		1.0g/kg		3.0g/kg
		Saline	Ethanol	Saline	Ethanol	Saline	Ethanol
Adolescent	Amygdala
	Central	100 ± 17.46	110.65 ± 15.52	100 ± 14.92	106.6 ± 17.70	100 ± 9.43	460.34 ± 36.55*
	Basolateral	100 ± 12.16	73.55 ± 8.52	100 ± 21.44	83.92 ± 13.87	100 ± 11.66	443 ± 27.54*
	N. Accumbens
	Core	100 ± 12.2	110.33 ± 14.85	100 ± 13.40	106.61 ± 12.35	100 ± 11.95	100 ± 15.50
	Shell	100 ± 18.55	75.29 ± 13.89	100 ± 17.14	91.69 ± 16.78	100 ± 10.11	100 ± 12.94
	mPFC	100 ± 7.35	101.47 ± 8.79	100 ± 6.31	104.26 ± 7.80	100 ± 3.86	176.60 ± 2.62*
	Dentate Gyrus	100 ± 5.18	93.6± 1.7	100 ± 8.29	56.29 ± 6.11*	100 ± 4.21	35.2 ± 4.64*
Adult	Amygdala
	Central	100 ± 17.77	127.75 ± 16.18	100 ± 16.10	128.32 ± 12.82	100 ± 18.49	513.81 ± 77.18*
	Basolateral	100 ± 23.06	53.01 ± 24.42	100 ± 27.03	127.08 ± 27.79	100 ± 9.22	121.38 ± 12.10
	N. Accumbens
	Core	100 ± 11.46	115.07 ± 18.62	100 ± 14.60	128.66± 18.66	100 ± 10.21	92.95 ± 15.55
	Shell	100 ± 30.28	87.53 ± 16.10	100 ± 22.96	93.64 ± 18.04	100 ± 22.24	97.37 ± 10.41
	mPFC	100 ± 8.91	96.92 ± 590.5	100 ± 7.61	105.84 ± 13.26	100 ± 5.48	139 ± 10.33*
	Dentate Gyrus	100 ± 6.33	103.54 ± 5.60	100 ± 5.82	89.39± 10.04	100 ± 3.28	38.33 ± 5.05*

^*sig diff from saline p<0.05