Table 1.

Mass spectrometric and RP-HPLC characterization of conjugates

Analog		Molecular Formula	MS Calculated	MS Observeda	RP-HPLC Retention Timeb	IC50c/nM
1	DOTA-8 AOC–BBN(8-14) NH2	C67H106N18O17S	1467.7	1468.0	14.99	10.2 ± 3.5
2*	DOTA [(D)K - 5- AVA]–BBN(8- 14) NH2	C70H112N20O18S	1553.8	1553.4	11.10	-
3*	DOTA [(D)K - (D)K -G] –BBN(8- 14) NH2	C73H118N22O19S	1639.8	1639.5	6.50	-
4*	DOTA [(D)K - (D)K - (D)K ] – BBN(8-14) NH2	C77H127N23O19S	1710.9	1711.3	9.0	-
2	DOTA [(D)K (2-NIAA)- 5- AVA] –BBN(8-14) NH2	C75H115N23O21S	1706.8	1707.1	12.05	10.8 ± 2.8
3	DOTA [(D)K (2-NIAA)- (D)K (2- NIAA)-G] –BBN(8-14) NH2	C83H124N28O25S	1945.9	1946.1	14.01	48.5 ± 27.1
4	DOTA [(D)K (2-NIAA)- (D)K (2- NIAA)- (D)K (2-NIAA) ] – BBN(8-14) NH2	C92H136N32O28S	2170.0	2170.4	14.76	1110 ± 205.0
In-1	In-DOTA-8 AOC–BBN(8-14) NH2	C67H103N18O17SIn	1580.5	1580.2	15.94	23.5 ± 15.9
In-2	In-DOTA [(D)K (2-NIAA)- 5- AVA] –BBN(8-14) NH2	C75H112N23O21SIn	1819.7	1819.5	12.67	5.70 ± 1.31
In-3	In-DOTA [(D)K (2-NIAA)- (D)K (2-NIAA)-G] –BBN(8-14) NH2	C83H121N28O25SIn	2058.9	2058.6	14.19	93.0 ± 37.3
In-4	In-DOTA [(D)K (2-NIAA)- (D)K (2-NIAA)- (D)K (2-NIAA) -G] – BBN(8-14) NH2	C92H133N32O28SIn	2283.1	2283.2	15.04	342 ± 99.0

^aFor convenient mass spectra analysis ¹¹¹In was replaced by ^natIn.

^bRP-HPLC methods described in Materials and Methods section.

^cValues represent mean ± SEM. (n=6)