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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Exp Hematol. 2011 Nov 25;40(3):228–237.e5. doi: 10.1016/j.exphem.2011.11.007

Figure 2. Rps29 mutant microarray reveals dose-dependent genes.

Figure 2

(A) Microarray schematic. Rps29+/- fish were incrossed, and mutant embryos were sorted at 24 hpf. RNA was isolated from sibling embryos and mutant embryos for microarray.

(B) Rps29 expression at the 8 somite stage by whole mount ISH.

(C) Pathways significantly affected in the rps29 mutant, identified by Ingenuity Pathway Analysis.

(D) Dose-dependent expression of otpa. Orthopedia protein a (otpa) levels were assayed by ISH at 24 hpf. Expression can be seen in the telencephalon and hindbrain.

(E) RT-PCR for otpa. Data shown are fold changes of expression in rps29+/- and rps29-/- embryos compared to wildtype siblings at 24 hpf.