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(A) Morphology of embryos at 24 hpf.
(B) Hemoglobin at 40-48 hpf measured by staining with o-dianisidine (benzidine).
(C) HSCs at 36 hpf measured by ISH for myb.
(D) Vasculature at 24 hpf assayed by ISH for flk1.
(E) Otpa expression at 24 hpf assayed by ISH.
