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. Author manuscript; available in PMC: 2013 Mar 5.
Published in final edited form as: Mol Pharm. 2012 Feb 13;9(3):634–644. doi: 10.1021/mp200571k

Figure 4.

Figure 4

Mitaplatin significantly triggered the dysfunction of mitochondria in CP-r cells. (A) Ultrastructural analysis of mitochondrial changes in KB-CP 20 cells was carried out by transmission electron microscopy. After mitaplatin treatment for 72 h, the mitochondria appeared more irregular. They were much smaller with more highly condensed shapes (arrow) and had zones of intercellular detachment (asterisk), compared to those in cells with cisplatin treatment. (B) Changes of mitochondrial membrane potential in KB-CP 20 and BEL 7404-CP 20 cells were examined by flow cytometry after treatment with 100 µM of various drugs. CCCP was used as a positive control. (C) Changes of mitochondrial membrane potential in KB-CP 20 and BEL 7404-CP 20 cells imaged by confocal microscopy. JC-1 existed either as a green fluorescent monomer at depolarized membrane potentials or as an orange-red fluorescent J-aggregate at hyperpolarized membrane potentials. The shift in membrane charge was observed by disappearance of fluorescent red-orange-stained mitochondria and an increase in fluorescent green-stained mitochondria. The ratio of J-monomer and J-aggregate form was increased after the treatment of mitaplatin for 72 h. The results were consistent with FACS quantitative measurements.