Figure 4. miR-34a inhibits tumor growth of osteosarcoma in vivo.
The results showed that the growth velocities of orthotopic tumors in miR-34a group (stable SOSP-9607 cells transfected with pcDNA-miR34a.) decreased as compared with control group (stable SOSP-9607 cells transfected with pcDNA3.1). (A) Representative photographs of tumors (arrows) on the left legs of mouse. (B) Representative photographs of orthotopic tumors harvested 42 days after inoculation. (C) Tumor growth curves measured after the inoculation. The length (L) and width (W) of tumor measured every 7 days after inoculation, and the volume of tumor was calculated according to the formula: volume = 1/2×L×W2. (D) Orthotopic tumor weights 42 days after inoculation. Data are presented as means±SD. (E) 42 days after inoculation, miR-34a expression levels in Orthotopic tumors were tested and showed in relative miR-34a levels. *P<0.05, ** P<0.01 (n = 6) are considered as statistically significant.