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. 2012 Mar 21;7(3):e33778. doi: 10.1371/journal.pone.0033778

Figure 6. c-Met is a target of miR-34a, and regulates the migration and invasion of osteosarcoma cells.

Figure 6

(A) Western blotting analysis of c-Met protein expression. (B) qRT-PCR analysis of c-Met mRNA expression. The mRNA levels of c-Met in three groups of SOSP-9607 cells (blank, SOSP-9607 cells; control, stable SOSP-9607 cells transfected with pcDNA3.1; miR-34a, stable SOSP-9607 cells transfected with pcDNA-miR34a.) were normalized against that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) which served as an internal control. (C) The result from luciferase assay showed that the luciferase activity of the pmiR-Met UTR-Wt construct was significantly inhibited after the introduction of miR-34a mimics. Meanwhile, mutations of the two c-Met 3′UTR-binding sites abolished the ability of miR-34a to regulate luciferase expression. (D, E) SOSP-9607 cells were transiently transfected with 100 nM of c-Met siRNA or inhibitor NC (Genepharma, China), respectively. 48 h later, the migration and invasion assay were performed. Quantitative results for the effects of c-Met siRNA on the migration and invasion ability of SOSP-9607 cells were shown as migrated and invaded cell number. The results are presented as means±SD. *P<0.01 (n = 3) is accepted as statistically significant.