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. 2012 Apr;40(4):726–733. doi: 10.1124/dmd.111.040329

Fig. 1.

Fig. 1.

Regulation of pIS-HNF4A by miRNAs. HeLa cells were cotransfected with the following. A, pIS-0 or pIS-HNF4A luciferase plasmids, along with the Renilla reporter plasmid for normalization. These cells were also transfected with miRNA Mimics. The data are expressed as the pIS-HNF4A luciferase activity corrected for Renilla luciferase and normalized to the negative control (cel-miR-67) within each experiment (mean ± S.E.M.; n = 3 independent experiments). B, pIS-HNF4A luciferase plasmid along with the Renilla reporter plasmid for normalization. These cells were also transfected with miRNA Mimics individually (cel-miR-67, hsa-miR-34a, hsa-miR-34b*, or hsa-miR-765; 30 nM) or together (hsa-miR-34b* + hsa-miR-34c-5p or hsa-miR-34c-5p + hsa-miR-765; 15 nM each). All data are expressed as pIS-HNF4A luciferase activity corrected for Renilla luciferase and normalized to the negative control (cel-miR-67) within each experiment (mean ± S.E.M.; n = 3 independent experiments). *, p < 0.05; **, indicates p < 0.001, compared with the negative control.