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. 2012 Apr;40(4):717–725. doi: 10.1124/dmd.111.042416

Fig. 2.

Fig. 2.

Efavirenz concentrations versus formation rate of 8-hydroxyefavirenz (A) and bupropion concentrations versus formation rate of 4-hydroxybupropion (B) in microsomes containing cDNA-expressed CYP2B6.1 and CYP2B6.6 without coexpression of Cyt b5. Efavirenz (1–200 μM) or bupropion (10–1000 μM) was incubated with reconstituted systems containing either CYP2B6.1 or CYP2B6.6 (15 pmol) and an NADPH-generating system for 15 min at 37°C. Formation rate of 8-hydroxyefavirenz and 4-hydroxybupropion (pmol/min/pmol P450) versus substrate concentrations were fit to the simple single-site Michaelis-Menten equation. Each point represents mean ± S.D. of three replicates. 8-OHEFV, 8-hydroxyefavirenz; 4-OHBUP, 4-hydroxybupropion.