Fig. 2.

MS/MS spectra and proposed origin of fragment ions for metabolites M1, M3, M4, and M5. HLMs (2 mg/ml) were incubated with 20 μM ETR for 30 min at 37°C in the presence of an NADPH-regenerating system. Metabolites were detected and fragmented using UPLC-MS/MS in product ion mode. Data shown are representative of three individual experiments.