Fig. 7.

Comparison of M3 and M4 formation by HLMs genotyped for CYP2C19. HLMs (0.5 mg/ml) genotyped as CYP2C19*1/*1, CYP2C19*1/*2, and CYP2C19*2/*2 were incubated with 20 μM ETR in the presence of an NADPH-regenerating system for 30 min at 37°C, and formation of M3 and M4 was measured using UPLC-MS in multiple reaction monitoring mode. Data are presented as the mean percentage of metabolite formation by CYP2C19*1/*1 HLMs. The assay was performed in triplicate. Statistical significance was determined by performing a Student's t test. **, p < 0.01.