Table 2.
Genetic characterization of all sampled populations in the Dutch Kuinderbos forest
| Species | Population | Pop. size | Yest | No. of samples | Ng | A | Ar | Ap/A | A/AKB | Ho | He | F | Founder |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ASPS | Asco1 (KB1) | 339 (238) | 1980–1985 | 9 | 1 | 1·00 | 1·00 | 0·00 | 0·15 | 0·000 | 0·000 | NA* | ? |
| Asco2 (KB2) | 206 (145) | 1975–1980 | 10 | 7 | 2·50 | 2·02 | 0·07 | 0·38 | 0·081 | 0·258 | 0·684 | ? | |
| Asco3 (KB4) | 328 (259) | 1975–1980 | 10 | 8 | 3·25 | 2·65 | 0·03 | 0·50 | 0·103 | 0·391 | 0·737 | ? | |
| Asco4 (KB10) | 260 (139) | 1970–1975 | 9 | 9 | 3·75 | 3·13 | 0·12 | 0·58 | 0·238 | 0·431 | 0·448 | ? | |
| Asco5 (KB12) | 262 (95) | 1970–1980 | 9 | 9 | 3·25 | 2·57 | 0·00 | 0·50 | 0·119 | 0·458 | 0·740 | ? | |
| Asco6 (KB13) | 149 (81) | 1970–1980 | 7 | 6 | 2·75 | 2·52 | 0·10 | 0·42 | 0·207 | 0·474 | 0·563 | ? | |
| ASPT | Atri1 | 10 (10) | About 1975 | 10 | 4 | 2·50 | NA | 0·18 | 0·67 | NA | 0·270 | NA | ? |
| Atri2 | 11 (11) | 1985–1990 | 11 | 2 | 1·75 | NA | 0·25 | 0·47 | NA | 0·385 | NA | ? | |
| Atri3 | 10 (10) | About 1995 | 10 | 5 | 2·25 | NA | 0·38 | 0·60 | NA | 0·431 | NA | ? | |
| POLS | Pset1 | 28 (3) | 1970–1980 | 10 | 1 | 1·00 | 1·00 | 0·00 | 0·16 | 0·000 | 0·000 | NA* | Yes |
| Pset2 | 60 (36) | 1980–1990 | 12 | 5 | 1·50 | 1·59 | 0·38 | 0·24 | 0·083 | 0·239 | 0·651 | Yes | |
| Pset3 | 29 (20) | 1970–1980 | 10 | 1 | 1·00 | 1·00 | 0·00 | 0·16 | 0·000 | 0·000 | NA* | ? | |
| Pset4 | 6 (1) | 2005–2010 | 4 | 4 | 1·75 | 1·63 | 0·13 | 0·28 | 0·125 | 0·208 | 0·4 | Yes | |
| Pset5 | 12 (8) | 1970–1980 | 10 | 10 | 3·75 | 2·39 | 0·53 | 0·60 | 0·369 | 0·431 | 0·143 | ? | |
| Pset6 | 5 (1) | 1985–1990 | 5 | 4 | 1·67 | 1·40 | 0·25 | 0·27 | 0·400 | 0·175 | –0·600 | No | |
| POLA | Pacu1 | 10 (2) | 1965–1975 | 7 | 3 | 2·00 | NA | 0·06 | 0·38 | NA | 0·192 | NA | Yes† |
| Pacu2 | 138 (36) | 1986 | 12 | 5 | 2·25 | NA | 0·08 | 0·43 | NA | 0·281 | NA | Yes† | |
| Pacu3 | 55 (40) | 1965–1975 | 11 | 1 | 1·00 | NA | 0·00 | 0·19 | NA | 0·000 | NA | Yes | |
| Pacu4 | 50 (>25) | 1965–1975 | 10 | 3 | 2·75 | NA | 0·13 | 0·52 | NA | 0·299 | NA | Yes† | |
| Pacu5 | 147 (>100) | 1965–1975 | 10 | 8 | 3·75 | NA | 0·00 | 0·71 | NA | 0·542 | NA | ? | |
| Pacu6 | 31 (>10) | 1985–1990 | 10 | 10 | 4·25 | NA | 0·15 | 0·81 | NA | 0·569 | NA | ? |
Species acronyms are as in Table 1. Asplenium scolopendrium populations overlap with those in Wubs et al. (2010) (codes used in that article are given in parentheses).
Pop. size, population size at the time of sampling (number of spore-bearing plants in parentheses); Yest, estimated time of initial population establishment (P. Bremer, unpubl. res.); Founder, the population founder homozygous at the tested loci [yes, no or founder unknown (?)].
Measures of genetic variation: Ng, number of multilocus genotypes; A, allelic variability (mean number of alleles per locus); Ar, allelic richness (number of alleles per locus, corrected for sample size by means of rarefaction); Ap/A, mean fraction of private alleles (within the Kuinderbos) per locus; A/AKB, fraction of the total allelic variability in the Kuinderbos that is present per population.
Measures of heterozygosity and inbreeding: Ho, observed heterozygosity (undefined for polyploids); He, expected heterozygosity; F, inbreeding coefficient (undefined for polyploids).
* Only one multilocus genotype present. The inbreeding coefficient is therefore undefined, but effectively equals 2 (extreme inbreeding).
† Most probably homozygous, but heterozygosity cannot be excluded as this (tetraploid) individual showed two alleles at some loci.