Table 1.

Classification, sources, AIR yields, and relative abundance of MLG subunits and xyloglucan of all species analysed

Classification	Species	Source/accession number*	Age†	Polymer content (mg g−1)‡	Relative oligosaccharide yields§
					MLG4	MLG3	MLG2	XGOs
HETEROSPOROUS LYCOPHYTE
Selaginellaceae	Selaginella willdenowii (Desv. ex Poir.) Baker	19762969(E)	Y	117	–	–	–	+
EUSPORANGIATE MONILOPHYTES
Ophioglossaceae	Ophioglossum vulgatum L.	19695522(E)	Y	67	–	–	–	+
Psilotaceae	Psilotum nudum (L.) P. Beauv.	20040835(E)	Y	45	–	–	–	+
			M	109	–	–	–	+
Equisetaceae¶	Equisetum bogotense Kunth.	NYBG herbarium	(H)	nd	++ +	–	–	[ ]
subgenus Equisetum	Equisetum arvense L.	RBGE	Y	91	++	±	+	+
			M	74	++ +	±	++	+
	Equisetum fluviatile L.	KB pond	Y	32	++	±	+	+
			M	nd	++ +	±	+	–
	Equisetum pratense Ehrh.	19821716(E)	Y	95	++	±	±	+
			M	129	++ +	±	±	+
	Equisetum sylvaticum L.	19821736(E)	Y	90	++	±	+	–
			M	164	++ +	±	+	[ ]
	Equisetum × bowmanii C.N. Page (E. telmateia × E. sylvaticum)	20060765A(E)	Y	129	++ +	–	++	–
			M	98	++ +	±	+	[ ]
	Equisetum × litorale Kühlewein ex Rupr. (E. arvense × E. fluviatile)	19821740(E)	Y	58	++ +	±	+	+
			M	97	++ +	±	+	+
	Equisetum × robertsii Dines (E. arvense × E. telmateia)	20060766A(E)	Y	81	++ +	+	++	++
			M	74	++	±	±	++
subgenus Hippochaete	Equisetum hyemale var. affine Engelm.	19734597B(E)	Y	35	+	–	–	++ +
			M	104	+	–	±	+
	Equisetum myriochaetum Schltdl. & Cham.	19734598(E)	Y	35	+	±	+	++
			M	100	++	±	±	+
	Equisetum ramosissimum ssp. debile Hauke	19731694(E)	M	nd	+	–	++ +	[ ]
Marattiaceae	Marattia fraxinea Sm.	19697184(E)	Y	68	–	–	–	++ +
			M	65	–	–	–	++ +
	Angiopteris evecta Hoffm.	20060955(E)	Y	82	–	–	–	++ +
			M	58	–	–	–	++
	Angiopteris lygodiifolia Rosenstock	19763705A(E)	Y	29	–	–	–	++ +
			M	57	–	–	–	++ +
LEPTOSPORANGIATE MONILOPHYTES
Osmundaceae	Osmunda regalis L.	19913878A(E)	Y	108	–	–	–	++ +
			M	203	–	–	–	+
	Todea barbara (L.) T. Moore	19652792(E)	Y	98	–	–	–	++ +
			M	134	–	–	–	+
Hymenophyllaceae	Trichomanes speciosum Willd.	19992144(E)	Y	191	–	–	–	++ +
			M	224	–	–	–	++
Dipteridaceae	Dipteris conjugata Reinw.	20021886A(E)	Y	145	–	–	–	+
			M	249	–	–	–	±
Lygodiaceae	Lygodium japonicum (Thunb.) Sw.	19734355(E)	Y	nd	–	–	–	++
			M	nd	–	–	–	+
Anemiaceae	Anemia sp.	19933657(E)	Y	116	–	–	–	++ +
			M	154	–	–	–	++
Marsileaceae	Marsilea drummondii A. Braun	19933710(E)	Y	100	–	–	–	+
			M	111	–	–	–	+
Thyrsopteridaceae	Thyrsopteris elegans Kunze	20031267A(E)	Y	108	–	–	–	++ +
			M	70	–	–	–	+
Cyatheaceae	Cyathea spinulosa Wall.	19941397A(E)	Y	87	–	–	–	++ +
			M	54	–	–	–	++
Woodsiaceae	Woodsia obtusa (Spr.) Torrey	20061094A(E)	Y	99	–	–	–	++ +
			M	73	–	–	–	++
SPERMATOPHYTES
Gymnosperm	Chamaecyparis lawsoniana Parl.	KB	Y	216	–	–	–	++
			M	255	–	–	–	++
Angiosperm	Nymphaea lotus L.	20040381(E)	Y	26	–	–	–	++ +
			M	278	–	–	–	++ +

* Sources/accession numbers: (E), Royal Botanic Garden, Edinburgh (RBGE) accession no.; KB, King's Buildings campus, Edinburgh; NYBG, New York Botanic Garden.

^† Age of tissues tested: Y, young tender tissues; M, mature tough tissues; H, herbarium tissue.

^‡ Polymer contents: yield of alcohol-insoluble residue (mg AIR g⁻¹ fresh weight); nd, not determined.

^§ Oligosaccharides indicative of MLG and xyloglucan: MLG4, MLG3, MLG2, tetra-, tri- and disaccharide of MLG released by lichenase; XGOs, xyloglucan oligosaccharides released by XEG. Yields of the oligosaccharides are scored as: –, undetectable; ±, inconsistently or barely detectable; +, ++ , + ++ , low, moderate and high abundance; [ ], not determined.

^¶ E. bogotense cannot be placed clearly in either subgenus.