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(a) The domain architecture and constructs used in these studies. (b) SDS–PAGE (13%) monitoring of the overexpression and purification of XccFimXEAL. Lane 1, protein markers (labelled in kDa); lane 2, whole cell lysate before IPTG induction; lane 3, whole cell lysate after IPTG induction; lane 4, supernatant of His6-tagged XccFimXEAL; lane 5, gel-purified XccFimXEAL after TEV cleavage.
