Figure 1.

Purification of the human Fyn SH2 domain. (a) Analytical gel-filtration profile of non-His-tagged Fyn SH2 domain on a Superdex 75 HR column (loaded onto the column at 14 mg ml⁻¹). The molecular-weight standards and protein chromatograms are represented as red and blue lines, respectively. The insert shows a 20% SDS–PAGE under reducing conditions of the molecular-weight marker and the purified Fyn SH2 domain. (b) Mass-spectrometric analysis of N-terminally His-tagged Fyn SH2 domain (from pColdII expression vector; expected mass based on the sequence, 13 231.0 Da). The insert shows 15% SDS–PAGE under reducing conditions of the molecular-weight marker and the purified Fyn SH2 domain.