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. 2005 Sep;11(9):1446–1448. doi: 10.3201/eid1109.050179

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This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.

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A) Distribution of HIV-1 Western blot–indeterminate patterns among 69 serum specimens from Kinshasa, Zaire, reactive by enzyme immunoassay. B) Phylogenetic classification of HIV-1 protease sequence ZA30972 (GenBank accession no. AY562558) isolated from the p17 gag-reactive serum. The phylogenetic tree was generated by the neighbor-joining method with the nucleotide distance calculated by Kimura 2-parameter method (12), included in the GeneStudio package (http://www.genestudio.com). Reference sequences were obtained from the Los Alamos database (http://hiv-web.lanl.gov/MAP/hivmap.html). The position of the outgroup (Simian immunodeficiency virus [SIV]cpz) is not shown. Values on branches represent the percentage of 100 bootstrap replicates. The scale bar indicates an evolutionary distance of 0.10 nucleotides per position in the sequence.