TABLE 2.

NNK-induced O⁶-mG adduct formation in the liver and lung

Two-month-old WT B6 and Cyp2a5-null female mice were injected with NNK at a dose of 20 or 100 mg/kg i.p., and tissue levels of O⁶-mG and total guanine were determined at 4 h after the injection, as described under Materials and Methods. Data represent means ± S.D. (n = 8).

NNK Dose	Strain	O6-mG Level
		Lung	Liver
		pmol/μmol guanine
20 mg/kg	WT (B6)	5.4 ± 0.5	115 ± 13
	Cyp2a5-null	3.3 ± 0.8a	108 ± 18
100 mg/kg	WT (B6)	23.6 ± 1.6	594 ± 70
	Cyp2a5-null	18.8 ± 1.7b	604 ± 48

^a p < 0.01, compared with WT mice; Student's t test.

^b p < 0.05, compared with WT mice; Student's t test.