TABLE 3.
Effects of 8-MOP on NNK-induced O6-mG adduct formation in the liver and lung of Cyp2a5-null mice
Two-month-old female Cyp2a5-null mice were pretreated with 8-MOP (at 0, 12.5, or 50 mg/kg, p.o.) in 0.2 ml of corn oil once daily for 3 consecutive days. One hour after the final injection of 8-MOP, NNK was given at a dose of 100 mg/kg i.p., and tissue levels of O6-mG and total guanine were determined at 4 h after the NNK injection. Data represent means ± S.D. (n = 4).
| 8-MOP Dose | O6-mG Level |
|
|---|---|---|
| Lung | Liver | |
| pmol/μmol guanine | ||
| 0 mg/kg | 19.0 ± 1.8 | 590 ± 21 |
| 12.5 mg/kg | 13.4 ± 1.5a | 337 ± 70b |
| 50 mg/kg | 6.2 ± 3.0b | 170 ± 99b |
a
p < 0.05, compared with the 0 mg/kg 8-MOP (vehicle control) group (one-way ANOVA followed by Tukey's post hoc test).
b
p < 0.01, compared with the 0 mg/kg 8-MOP (vehicle control) group (one-way ANOVA followed by Tukey's post hoc test).