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. 2012 Apr;341(1):104–114. doi: 10.1124/jpet.111.187385

Fig. 6.

Fig. 6.

Effects of PI3 kinase inhibitors 8 to 11 on IFN-γ-treated T84 monolayers. Cells were treated with IFN-γ for 48 h in the presence of isozyme-specific PI3K inhibitors. The flux of Cy3-D8mer peptide and the incorporation of 5BP were measured as described under Materials and Methods. A and B, the effect of compound 15e (8) on IFN-γ-induced peptide permeability (A) and TG2 activation (B). C and D, the effect of TGX-221 (9) on IFN-γ-induced peptide permeability (C) and TG2 activation (D). Elevated concentrations of TGX-221 (≥10 μM) increased peptide permeability, likely due to toxicity. E and F, the effect of AS-252424 (10) on IFN-γ-induced peptide permeability (E) and TG2 activation (F). G and H, the effect of IC-87114 (11) on IFN-γ-induced peptide permeability (G) and TG2 activation (H). DMSO controls show no influence on peptide flux or TG2 activity. Data shown are normalized to the 0 U/ml IFN-γ condition represented by mean ± S.D.