Fig. 2.

HDAC inhibition stimulates endogenous cardiac regeneration after myocardial infarction. ICR mice were subjected to MI by ligation of the left descending coronary artery for 8 weeks. Trichostatin A and 5-bromo-2-deoxyuridine were administrated. Detailed methods of producing myocardial infarction and delivery of HDAC inhibitor and BrdU are under Materials and Methods. Quantitative analyses of BrdU (A and B), c-kit⁺ CSC (C), Ki67 (D), and pH3 (E) staining in paraffin-embedded sections are shown. Hearts were fixed and sectioned 8 weeks after MI. Values represent mean ± S.E. (n = 3–4/per group). *, p < 0.05.