Figure 6.
Fasudil increases endplate (EP) area in the tibialis anterior (TA) and transversus abdominis (TVA) muscles. Muscles were isolated from post-natal (P) day 21 untreated control littermates (n = 3), vehicle-treated Smn2B/- (n = 4) and fasudil-treated Smn2B/- mice (n = 3). (A) Representative images of TA and TVA EPs stained with α-bungarotoxin (BTX). Scale bars = 25 μm (TA) and 30 μm (TVA). (B) Quantification of EP area shows that fasudil-treated Smn2B/- TA and TVA muscles display significantly larger EPs when compared to vehicle-treated Smn2B/- muscles. (***P < 0.001; ****P < 0.0001; data are mean +/- s.d.).