Figure 3. LHR orthologs have conserved localization properties despite species-specific divergence of heterochromatin.

(A,B) The dodeca satellite has diverged in its chromosomal location and interphase organization between D. melanogaster and D. simulans. FISH to mitotic (A) and interphase (B) nuclei from 3^rd instar larval brain cells with probes to dodeca (green) and 2L3L (red). Right panels in part B show quantification of the nuclear distribution of the interphase dodeca FISH signals. The mean values are indicated by the green lines (n = 10 for each sample).Boxes span the interquartile range and whiskers extend to the maximum and minimum values. Significance was tested by Wilcoxon rank-sum test. mel = D. melanogaster; sim = D. simulans. (C, D) Conserved heterochromatic localization properties of LHR orthologs. (C) sim-Lhr-HA transgene in D. simulans embryos. Top panel, Anti-HA (green) detects sim-LHR-HA colocalizing with HP1 (red) in the apical heterochromatin. Bottom panel, sim-LHR-HA (green) partially colocalizes with dodeca satellite (blue). (D) sim-LHR-HA (green) expressed in D. melanogaster embryos colocalizes with H3K9me2 (red) and with mel-LHR-YFP, detected with anti-GFP (red). sim-LHR-HA also partially overlaps with the D. melanogaster dodeca satellite (blue).