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. 2012 Mar 22;8(3):e1002597. doi: 10.1371/journal.pgen.1002597

Figure 6. Asymmetric expression of Lhr orthologs in hybrids.

Figure 6

Pyrosequencing across a SNP fixed between Lhr orthologs was used to measure the ratio of allelic transcription in pure-species and hybrid larvae that were 3–5 days old. Lhr transcript from pure species D. melanogaster is 100% for the D. melanogaster-specific variant of the SNP. For D. simulans 2 out of 3 of the technical replicates from one of the cDNA preparations showed 100% of transcripts with the D. simulans-specific variant of the SNP; a small amount of the D. melanogaster-specific SNP detected in the 3rd replicate most likely reflects error or contamination. The D. simulans-specific SNP is detected at levels significantly greater than the expected 50% in both male and female hybrids (p<.0001 and p = .005 for hybrid males and females, respectively, by two-tailed t-test).