Table 1. D. melanogaster and D. simulans Lhr orthologs suppress hybrid rescue by D. simulans Lhr.¹ .

					No. of hybrid males
Cross	Transgenic construct	attP integration site	Temp.	No. of hybrid females	Genotype 1 +/+	Genotype 2 φ{ }/+ (or attP2/+)
1	φ{sim-Lhr-HA}	attP2	RT	232	92	0
			18°C	214	110	0
2	φ{sim-Lhr-HA}	86Fb	RT	135	74	0
			18°C	100	57	0
3	φ{mel-Lhr-HA}	attP2	RT	177	91	0
			18°C	240	122	0
4	φ{mel-Lhr-HA}	86Fb	RT	263	121	0
			18°C	246	109	0
5	φ{sim-Lhr}	attP2	RT	184	61	0
6	φ{mel-Lhr}	attP2	RT	302	150	0
			18°C	217	84	0
7	φ{ΔBap55, mel-Lhr-HA}	attP2	RT	324	156	0
			18°C	322	188	0
8	none	attP2	RT	280	NA	160

Crosses were between D. melanogaster females heterozygous for the different transgenes tested (w; ϕ{transgene, w⁺}) and D. simulans Lhr¹ males. Transgenes are denoted as φ{} in the table. The transgenes carry a copy of the w⁺ gene so the hybrid sons inheriting the transgene (genotype 2) were distinguished from their control siblings (genotype 1) by their eye-colour, except for cross 8 where D. melanogaster females homozygous for the integration site without an inserted transgene were mated to D. simulans Lhr¹ males. NA = not applicable. RT = room temperature.