Table 3. Rescue of D. melanogaster/D. mauritiana hybrid male lethality by D. melanogaster Lhr− deletions.
No. of F1 hybrid females | No. of F1 hybrid males | ||||||
Deletion tested | D. mauritiana male parent | Df/+ | Balancer/+ | Df/+ | Balancer/+ | Pharate adult | Estimated pharate male viability |
l(2)k01209 | Iso 105 | 54 | 35 | 0 | 0 | 4 | 7.4% |
W139 | 291 | 305 | 0 | 1 | 24 | 8.2% | |
Df(2R)BSC49 | Iso 105 | 70 | 63 | 0 | 0 | 15 | 21.4% |
W139 | 142 | 35 | 0–3a | 0–3a | 0 | 0 | |
Df(2R)BSC44 | w f | 47 | 44 | 0 | 0 | 0 | 0 |
Iso 105 | 87 | 85 | 0 | 0 | 0 | 0 | |
W139 | 60 | 80 | 0 | 0 | 0 | 0 | |
Other | various | 6643 | 6589 | 12 | 8 | 0 | 0 |
D. melanogaster females with the designated deletions were mated to D. mauritiana males at 18°. The small number of live males recovered are likely patroclinous exceptions, that is Xmau/O. Pharate adults were scored after all adults had eclosed. “Estimated pharate male viability” was calculated as number of pharate adult males/number of Df/+ females. For crosses with l(2)k01209, pharate adult males displayed the orange-eye color characteristic of Df/+. For crosses with Df(2R)BSC49, the genotypes of the pharate adults could not be determined and were thus assumed to be Df/+. Full genotypes of Lhr− deletions are: y1,w67c23; P{w+mC = lacW}l(2)k01209[k08901a]/CyO; Df(2R)BSC49/SM6a; and Df(2R)BSC44/SM6a. All three were previously shown to be deleted for Lhr [16]. “Other” refers to 45 different Lhr+ deletions spanning chromosome arm 2R.
Cross produced 3 total males, which were not genotyped for Df or balancer.