Skip to main content
. 2012 Mar 22;7(3):e31914. doi: 10.1371/journal.pone.0031914

Figure 6. Localization of eimeripain_mCHER within E. tenella.

Figure 6

Sporozoites were transfected with the plasmid pEimerip/Eimerip_mCherry and directly inoculated into chickens via the cloaca to complete the Eimeria life cycle in vivo. Seven days post-inoculation, oocysts were harvested from the caeca. mCherry+parasites (red) were observed with a Zeiss Axiovert 200 microscope equipped with the Apotome imaging system. (A) Localization of eimeripain_mCHER in unsporulated oocysts (USOO) and sporulated oocysts (SOO). (B) Localization of eimeripain_mCHER in extracellular sporozoites. Nuclei of sporozoites were stained with DAPI (blue). Two examples of eimeripain_mCHER expressing sporozoites are shown. (C) Localization of eimeripain_mCHER in intracellular sporozoites. Infected MDBK cells were fixed and observed 24 h post invasion by recombinant sporozoites. Nuclei of host cells are detected with DAPI (blue). Two examples of intracellular eimeripain_mCHER expressing sporozoites are shown. The posterior refractile body is indicated by an asterisk. The scale bar represents 2 µm.