Figure 2. HIV-1 suppresses lymphocyte translation but HIV-1 gag translation is sustained.

(A) Following HIV-1 infection and treatment with nocodazole (Noc) or cyclohexamide (CHX), CEMx174 cells were incubated with [³⁵S]-cysteine/methionine for one hour and proteins were precipitated with TCA. [³⁵S]-cysteine/methionine incorporation was measured by scintillation in three independent experiments and is presented with standard deviation. Asterisk indicates the significant reduction, p<0.05. Propidium iodide staining intensity and ModFit analysis demonstrates the number and percentage of cells in the G1 or G2/M phase of the cell cycle. Percentages are summarized below the plots. (B) CEMx174 cells received mock, nocadozole or CHX treatment. TCA precipitable counts and the cell cycle profiles were measured as described in (A). (C) Equivalent whole-cell extracts were resolved by SDS-PAGE and labeled proteins were detected by the phosphorimager analysis. Percentage cellular translation relative to the mock treatment or infection is summarized below the gel. Equivalent whole-cell extracts were subjected to immunoprecipitation after metabolic labeling and proteins were resolved by SDS-PAGE and phosphorimager analysis is presented. Positions of Gag p55, p37 and p24 are indicated.