Figure 4. HIV-1 gag mRNA translation is resistant to suppression of global cellular translation.

CEMx174 cells were infected with HIV-1 or ΔVifVprX (ΔVV) and evaluated at 12 hour intervals post-infection. At indicated intervals, replicate cultures were metabolically labeled with [³⁵S]-cysteine/methionine for one hour and proteins immunoprecipitated with antisera to Gag and Actin. The immunoprecipitated proteins were resolved by SDS-PAGE and phosphorimager analysis is presented. Positions of Actin and Gag p55, p37 and p24 are indicated. In parallel, RNA was harvested and RT-qPCR with gag or actin primers was performed to measure RNA copy numbers. The ratio of the RNA copy numbers is presented.