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. 2012 Mar 22;8(3):e1002612. doi: 10.1371/journal.ppat.1002612

Figure 6. Suppression of cellular translation is attributable to HIV-1-dependent dephosphorylation of eIF4E and 4E-BP1.

Figure 6

(A) HEK 293 cells were transfected with HIV-1NL4-3 or ΔVifVprX (ΔVV) for 24 or 48 hour, as indicated. Equivalent whole-cell extracts were immunoblotted with antiserum against phospho-eIF2α Ser51, total eIF2α, phospho-eIF4E Ser209 or total eIF4E, respectively. (B) CEMx174 cells were infected with HIV-1 or ΔVifVprX (ΔVV) and evaluated at 12 hour intervals. Equivalent whole-cell extracts were immunoblotted with the indicated antiserum.