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. 2012 Mar 22;8(3):e1002612. doi: 10.1371/journal.ppat.1002612

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Sharma et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Cellular protein and cell-free supernatant medium from CEMx174 cells or CEMx174/HIV-1 were harvested and subjected to immunoblot. Virions in the medium were collected by ultracentrifugation, subjected to Gag ELISA and the indicated aliquots (0 to 2000 ng) were evaluated with the indicated antisera.