Skip to main content
. 2012 Mar 22;7(3):e33195. doi: 10.1371/journal.pone.0033195

Figure 5. Intercellular transfer of small molecules via a TNT of ARPE-19 cells.

Figure 5

(A) The DIC image show a membrane tube (arrow) and two connected ARPE-19 cells (1, 2). (B-C) Cell 1 and Cell 3 were injected with a mixture of Lucifer yellow (green, B) and Texas red Dextran 10,000 (red, C). The fluorescence images taken 10 min after injection show that only Lucifer yellow was transmitted to cell 2 (green, B), while Texas red Dextran was not detected in cell 2 (dashed area in C). A typical result was shown from 5 independent experiments. Scale bar, 20 µm. (D) The overlay of DIC, the Lucifer yellow-positive cells and the Texas red Dextran positive cells, and the membrane tube (arrow in A).