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. 2012 Mar 22;7(3):e33846. doi: 10.1371/journal.pone.0033846

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Xu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A, BEAS-2B cells were transfected with MUC1 siRNA or negative control siRNA. The cells were then treated with BPDE (0.1 µM) for 1 wk and seeded in soft agar. Colony formation was photographed under a light microscope. B, Quantitative representation of the transformation experiment. Bars show the averages of colony numbers of 6 randomly selected fields. Data shown are mean ± S.D; ** P<0.01. Insert, Confirmation of MUC1 knockdown by Western blot.