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. Author manuscript; available in PMC: 2013 Mar 2.
Published in final edited form as: J Proteome Res. 2012 Feb 15;11(3):1512–1520. doi: 10.1021/pr2003165

Figure 3. RP-HPLC of hydroxylamine-treated peptide 681 (ASEHATYG).

Figure 3

(A) iTRAQ-modified peptide. This HPLC run was performed at a different time than Fig. 2B, thus the peak RTs are somewhat different. (B) Modified peptide treated with hydroxylamine. Analysis of the large peak eluting at 42.6 min for remaining iTRAQ modification(s) by MS showed only singly modified peptide.