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. Author manuscript; available in PMC: 2013 Mar 21.
Published in final edited form as: J Am Chem Soc. 2012 Mar 7;134(11):5149–5156. doi: 10.1021/ja209868g

Figure 1. mbGuc-biotin reporter.

Figure 1

Schematic overview of multimodal imaging of the mbGluc-biotin reporter system. Upon vector-mediated delivery and expression, GFP is expressed. Biotin ligase will metabolically tag the BAP sequence within the reporter with a single biotin moiety followed by processing through the secretory pathway leading to displaying both Gluc and biotin on the cell surface. The biotin serves as a “molecular beacon” which can attract any imaging agent conjugated to streptavidin suited for fluorescence-mediated tomography (FMT), intravital microscopy (IVM), magnetic resonance (MR) and radionuclide imaging such as single emission photon computed tomography (SPECT) or positron emission tomography (PET). Further, the presence of Gluc on the cell surface allows bioluminescence imaging of this reporter.